Hotspot

psbA-trnK-UUU

LSC · 1437-1929 · 493 bp

Back to Dalbergia

Pi 0.049

MarkerSeek score 78.60

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 493
variable_sites: 127
pi: 0.049
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	547-661 bp	1.0	84.4
psbA-trnK-UUU_p2	GCTCTCGCGTCTCTCTAAAA	ACTCCATCCGACTAGTTCCG	50.0	55.0	57.5	58.6	496-610 bp	1.0	82.8
psbA-trnK-UUU_p3	ACGGTTTTCAGTGCTGGTTA	ACTAGTTCCGGGTTCGAGTC	45.0	55.0	57.7	58.8	537-651 bp	1.0	82.7
psbA-trnK-UUU_p4	ACGGTTTTCAGTGCTGGTTA	GACTAGTTCCGGGTTCGAGT	45.0	55.0	57.7	58.8	538-652 bp	1.0	82.7
psbA-trnK-UUU_p5	TACAGAAGCGACCCCATAGG	ACTCCATCCGACTAGTTCCG	55.0	55.0	58.6	58.6	521-635 bp	1.0	82.4