Hotspot

rps15-ycf1

SSC · 122368-122761 · 394 bp

Back to Dahlstedtia

Pi 0.0431

MarkerSeek score 68.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 394
variable_sites: 17
pi: 0.0431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TTTTTCTTGTGAAATAACTGGT	ACCCATAATGGAAGCCGTTT	27.3	45.0	52.4	57.5	502-510 bp	1.0	42.7
rps15-ycf1_p2	TTTTTCTTGTGAAATAACTGGT	CCCATAATGGAAGCCGTTTC	27.3	50.0	52.4	56.8	501-509 bp	1.0	42.7
rps15-ycf1_p3	TTTTTCTTGTGAAATAACTGGT	TACCCATAATGGAAGCCGTT	27.3	45.0	52.4	56.6	503-511 bp	1.0	42.7
rps15-ycf1_p4	TTTTTCTTGTGAAATAACTGGTA	ACCCATAATGGAAGCCGTTT	26.1	45.0	52.5	57.5	502-510 bp	1.0	42.7
rps15-ycf1_p5	TTTTTCTTGTGAAATAACTGGT	ACCCATAATGGAAGCCGTTTC	27.3	47.6	52.4	58.6	502-510 bp	1.0	42.7