Hotspot

ndhG-ndhI

SSC · 117333-117782 · 450 bp

Back to Dahlstedtia

Pi 0.0567

MarkerSeek score 70.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 450
variable_sites: 20
pi: 0.0567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GAGCCACGATAATTGCACCT	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.3	57.9	974-1120 bp	1.0	82.8
ndhG-ndhI_p2	GAGCCACGATAATTGCACCT	ACCGGTGTCAGGAATTGATG	50.0	50.0	58.3	57.9	951-1097 bp	1.0	82.7
ndhG-ndhI_p3	GAGCCACGATAATTGCACCT	TGGTCAACAAACAGTCCGAG	50.0	50.0	58.3	58.1	1353-1499 bp	1.0	82.6
ndhG-ndhI_p4	GAGCCACGATAATTGCACCT	CTGCGAGGTACATTGGTCAA	50.0	50.0	58.3	57.9	1333-1479 bp	1.0	82.3
ndhG-ndhI_p5	GAGCCACGATAATTGCACCT	CGTTTACCGGTGTCAGGAAT	50.0	50.0	58.3	57.9	1102 bp	0.5	56.6