Hotspot

ccsA-ndhD

SSC · 113793-114092 · 300 bp

Back to Dahlstedtia

Pi 0.0351
MarkerSeek score 70.40
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
300
variable_sites
10
pi
0.0351
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 AGTAGTGGCTTCTATGGGCT TCGGCTAATAAAGTTGAGGCT 50.0 42.9 57.8 57.1 432-445 bp 1.0 67.8
ccsA-ndhD_p2 CAGTAGTGGCTTCTATGGGC TCGGCTAATAAAGTTGAGGCT 55.0 42.9 57.8 57.1 433-446 bp 1.0 67.3
ccsA-ndhD_p3 AGTGGCTTCTATGGGCTTTC TCGGCTAATAAAGTTGAGGCT 50.0 42.9 57.6 57.1 429-442 bp 1.0 65.9
ccsA-ndhD_p4 GTGGCTTCTATGGGCTTTCT TCGGCTAATAAAGTTGAGGCT 50.0 42.9 57.6 57.1 428-441 bp 1.0 65.9
ccsA-ndhD_p5 AGTAGTGGCTTCTATGGGCT TTCGGCTAATAAAGTTGAGGCT 50.0 40.9 57.8 57.8 433-446 bp 1.0 65.4