Hotspot

rpl32-trnL-UAG

SSC · 112248-112641 · 394 bp

Back to Dahlstedtia

Pi 0.0604

MarkerSeek score 76.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 394
variable_sites: 23
pi: 0.0604
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGCAATCGATTTTTACGGGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	492-501 bp	1.0	84.0
rpl32-trnL-UAG_p2	CGCAATCGATTTTTACGGGG	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.9	57.8	485-494 bp	1.0	83.6
rpl32-trnL-UAG_p3	CGCAATCGATTTTTACGGGG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.9	57.8	490-499 bp	1.0	83.5
rpl32-trnL-UAG_p4	CGCAATCGATTTTTACGGGG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.9	57.8	491-500 bp	1.0	83.4
rpl32-trnL-UAG_p5	CGCAATCGATTTTTACGGGG	GAGCAGCGTGTCTACCAATT	50.0	50.0	57.9	58.3	486-495 bp	1.0	82.9