Hotspot

ycf1-ndhF

SSC · 109118-109124 · 7 bp

Back to Dahlstedtia

Pi 0.143

MarkerSeek score 72.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 7
variable_sites: 1
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GCTCGGGTTATGGAAGAAGG	ATATATGGGAGGTGGGCGAA	55.0	50.0	58.0	58.0	402 bp	1.0	80.7
ycf1-ndhF_p2	GCTCGGGTTATGGAAGAAGG	TATATGGGAGGTGGGCGAAT	55.0	50.0	58.0	58.0	401 bp	1.0	80.7
ycf1-ndhF_p3	GCTCCGTTCCATGTCTCATT	ATATATGGGAGGTGGGCGAA	50.0	50.0	58.0	58.0	800 bp	1.0	80.5
ycf1-ndhF_p4	GCTCCGTTCCATGTCTCATT	TATATGGGAGGTGGGCGAAT	50.0	50.0	58.0	58.0	799 bp	1.0	80.5
ycf1-ndhF_p5	GCTCCGTTCCATGTCTCATT	AATATATGGGAGGTGGGCGA	50.0	50.0	58.0	58.0	801 bp	1.0	80.4