Hotspot

rpl33-rps18

LSC · 67049-67242 · 194 bp

Back to Dahlstedtia

Pi 0.12
MarkerSeek score 75.90
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
194
variable_sites
23
pi
0.12
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 TCTGTCCCTGTTGTTGCAAA AGAAAGAGTCGTTTAGATTTATCCA 45.0 32.0 57.9 56.1 270-288 bp 1.0 47.2
rpl33-rps18_p2 TCTGTCCCTGTTGTTGCAAA GAAAGAGTCGTTTAGATTTATCCA 45.0 33.3 57.9 54.7 269-287 bp 1.0 47.2
rpl33-rps18_p3 TTCTGTCCCTGTTGTTGCAA GAAAGAGTCGTTTAGATTTATCCA 45.0 33.3 57.9 54.7 270-288 bp 1.0 47.2
rpl33-rps18_p4 TTCTGTCCCTGTTGTTGCAA AGAAAGAGTCGTTTAGATTTATCCA 45.0 32.0 57.9 56.1 271-289 bp 1.0 47.1
rpl33-rps18_p5 ATTCTGTCCCTGTTGTTGCA AGAAAGAGTCGTTTAGATTTATCCA 45.0 32.0 57.6 56.1 272-290 bp 1.0 47.1