Hotspot

trnR-UCU-trnG-UCC

LSC · 51543-51848 · 306 bp

Back to Dahlstedtia

Pi 0.0538

MarkerSeek score 78.50

Primer available yes

Label trnR-UCU-trnG-UCC

Feature Metadata

label_name: trnR-UCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 306
variable_sites: 15
pi: 0.0538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-UCC_p1	TGCAAATTTTCTGCGTCCAA	AGCCTTCCAAGCTAACGATG	40.0	50.0	57.1	58.0	406-433 bp	1.0	79.6
trnR-UCU-trnG-UCC_p2	TGCAAATTTTCTGCGTCCAA	CCTAGCCTTCCAAGCTAACG	40.0	55.0	57.1	58.1	409-436 bp	1.0	79.3
trnR-UCU-trnG-UCC_p3	ATGCAAATTTTCTGCGTCCA	AGCCTTCCAAGCTAACGATG	40.0	50.0	56.9	58.0	407-434 bp	1.0	77.8
trnR-UCU-trnG-UCC_p4	ATGCAAATTTTCTGCGTCCA	CCTAGCCTTCCAAGCTAACG	40.0	55.0	56.9	58.1	410-437 bp	1.0	77.5
trnR-UCU-trnG-UCC_p5	TGCAAATTTTCTGCGTCCAA	CCCTAGCCTTCCAAGCTAAC	40.0	55.0	57.1	57.7	410-437 bp	1.0	77.1