Hotspot

trnT-GGU-trnE-UUC

LSC · 29414-30226 · 813 bp

Back to Dahlstedtia

Pi 0.0274
MarkerSeek score 68.30
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
813
variable_sites
22
pi
0.0274
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GAACCGATGACTTACGCCTT ACTGCCTTTCGTAGTACCCT 50.0 50.0 58.0 58.1 957-960 bp 1.0 82.3
trnT-GGU-trnE-UUC_p2 CCATGGCGTTACTCTACCAC ACTGCCTTTCGTAGTACCCT 55.0 50.0 58.1 58.1 936-939 bp 1.0 81.9
trnT-GGU-trnE-UUC_p3 TGGCGTTACTCTACCACTGA ACTGCCTTTCGTAGTACCCT 50.0 50.0 58.1 58.1 933-936 bp 1.0 81.7
trnT-GGU-trnE-UUC_p4 GAACCGATGACTTACGCCTT TTTCGTAGTACCCTACCCCC 50.0 55.0 58.0 57.8 951-954 bp 1.0 81.3
trnT-GGU-trnE-UUC_p5 CCGATGACTTACGCCTTACC ACTGCCTTTCGTAGTACCCT 55.0 50.0 58.2 58.1 954-957 bp 1.0 81.1