Hotspot

trnV-UAC-ndhC

LSC · 10286-10647 · 362 bp

Back to Dahlstedtia

Pi 0.0525

MarkerSeek score 70.10

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 362
variable_sites: 19
pi: 0.0525
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	GTTCGAGTCCGTATAGCCCT	TCATATTCGTAAAGCAGAAACA	55.0	31.8	58.7	53.9	406-425 bp	1.0	43.7
trnV-UAC-ndhC_p2	AGAAGGTCTACGGTTCGAGT	TCATATTCGTAAAGCAGAAACA	50.0	31.8	57.8	53.9	418-437 bp	1.0	43.6
trnV-UAC-ndhC_p3	GAAGGTCTACGGTTCGAGTC	TCATATTCGTAAAGCAGAAACA	55.0	31.8	57.5	53.9	417-436 bp	1.0	43.6
trnV-UAC-ndhC_p4	AGAAGGTCTACGGTTCGAGT	TCATATTCGTAAAGCAGAAACAT	50.0	30.4	57.8	54.5	418-437 bp	1.0	43.6
trnV-UAC-ndhC_p5	AGAAGGTCTACGGTTCGAGT	ATCATATTCGTAAAGCAGAAACA	50.0	30.4	57.8	54.5	419-438 bp	1.0	43.5