Hotspot

ycf1

IRb · 108144-108707 · 564 bp

Back to Dahlia

Pi 0.00177
MarkerSeek score 44.00
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRb
length_bp
564
variable_sites
1
pi
0.00177
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CCCGGAACTTCTTCGTAGTG GGGGGTGGACGTATTTCTTC 55.0 55.0 58.0 58.0 969-971 bp 1.0 80.9
ycf1_p2 CCGGAACTTCTTCGTAGTGG GGGGGTGGACGTATTTCTTC 55.0 55.0 58.0 58.0 968-970 bp 1.0 80.9
ycf1_p3 GAGCTACTGAGGAACAACGG GGGGGTGGACGTATTTCTTC 55.0 55.0 58.0 58.0 1064-1066 bp 1.0 80.8
ycf1_p4 GGCAGAATAGCAGAGCAAGT GGGGGTGGACGTATTTCTTC 50.0 55.0 58.0 58.0 1362-1364 bp 1.0 80.4
ycf1_p5 GTCCCCCATCAGCGTATTTT GGGGGTGGACGTATTTCTTC 50.0 55.0 57.9 58.0 1422-1424 bp 1.0 80.3