Hotspot

rps15-ycf1

SSC · 122197-122598 · 402 bp

Back to Dahlia

Pi 0.015
MarkerSeek score 57.60
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
402
variable_sites
6
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AGTCTCCGTATCTTATTGGTGA GGGAGCCGCTTTAGTATGTT 40.9 50.0 56.1 57.7 557 bp 1.0 47.2
rps15-ycf1_p2 AGTCTCCGTATCTTATTGGTGA ATGGGAGCCGCTTTAGTATG 40.9 50.0 56.1 57.4 559 bp 1.0 45.4
rps15-ycf1_p3 AGTCTCCGTATCTTATTGGTGA CCAATAATGGGAGCCGCTTT 40.9 50.0 56.1 58.6 565 bp 1.0 45.0
rps15-ycf1_p4 AAGTCTCCGTATCTTATTGGTGA GGGAGCCGCTTTAGTATGTT 39.1 50.0 56.8 57.7 558 bp 1.0 44.9
rps15-ycf1_p5 AGTCTCCGTATCTTATTGGTGAA GGGAGCCGCTTTAGTATGTT 39.1 50.0 56.8 57.7 557 bp 1.0 44.9