Hotspot

ccsA-ndhD

SSC · 114097-114370 · 274 bp

Back to Dahlia

Pi 0.0185
MarkerSeek score 64.10
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
274
variable_sites
5
pi
0.0185
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GTTGTGGCAACTCTAGGCTT AGGTATTGGTATGTACCCCGA 50.0 47.6 58.1 57.7 464-467 bp 1.0 69.9
ccsA-ndhD_p2 TGTGGCAACTCTAGGCTTTC AGGTATTGGTATGTACCCCGA 50.0 47.6 57.8 57.7 462-465 bp 1.0 69.2
ccsA-ndhD_p3 GTTGTGGCAACTCTAGGCTT TCAGTTGACAAGGTTGAAGGT 50.0 42.9 58.1 57.4 427-430 bp 1.0 67.5
ccsA-ndhD_p4 GTGGCAACTCTAGGCTTTCT AGGTATTGGTATGTACCCCGA 50.0 47.6 57.5 57.7 461-464 bp 1.0 66.9
ccsA-ndhD_p5 TGTGGCAACTCTAGGCTTTC TCAGTTGACAAGGTTGAAGGT 50.0 42.9 57.8 57.4 425-428 bp 1.0 66.9