Hotspot

ycf1-ndhF

SSC · 108708-108782 · 75 bp

Back to Dahlia

Pi 0.087
MarkerSeek score 74.00
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
75
variable_sites
6
pi
0.087
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 CTAGCACTGGGTAGACCTCA GGGGGTGGACGTATTTCTTC 55.0 55.0 57.9 58.0 472-474 bp 1.0 81.1
ycf1-ndhF_p2 GCCGAGAAGAAGGTATCAGC GGGGGTGGACGTATTTCTTC 55.0 55.0 58.1 58.0 556-558 bp 1.0 80.8
ycf1-ndhF_p3 TCGTTGTGGTCGGACTCTAT GGGGGTGGACGTATTTCTTC 50.0 55.0 58.2 58.0 660-662 bp 1.0 80.2
ycf1-ndhF_p4 ATCAATTCGGTCGTTGTGGT GGGGGTGGACGTATTTCTTC 45.0 55.0 57.8 58.0 670-672 bp 1.0 80.1
ycf1-ndhF_p5 GCCGAGAAGAAGGTATCAGC TCGGGGTTACATAGATGCCT 55.0 50.0 58.1 57.9 713-715 bp 1.0 79.8