Hotspot

rpl22-rps19

LSC · 83421-83501 · 81 bp

Back to Dahlia

Pi 0.038
MarkerSeek score 76.60
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
81
variable_sites
3
pi
0.038
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TTACTCTTCGCGCTTTGTGA AGGAGAATTTGCGCCTACTT 45.0 45.0 57.9 57.2 225-227 bp 1.0 74.3
rpl22-rps19_p2 GAACGCCCACGAATTTGATC AGGAGAATTTGCGCCTACTT 50.0 45.0 57.8 57.2 247-249 bp 1.0 73.9
rpl22-rps19_p3 TACTCTTCGCGCTTTGTGAG AGGAGAATTTGCGCCTACTT 50.0 45.0 58.3 57.2 224-226 bp 1.0 73.0
rpl22-rps19_p4 TTACTCTTCGCGCTTTGTGA AGGAGAATTTGCGCCTACTTT 45.0 42.9 57.9 57.9 225-227 bp 1.0 72.0
rpl22-rps19_p5 GAACGCCCACGAATTTGATC AGGAGAATTTGCGCCTACTTT 50.0 42.9 57.8 57.9 247-249 bp 1.0 71.6