Hotspot

accD-psaI

LSC · 58241-58918 · 678 bp

Back to Dahlia

Pi 0.00737
MarkerSeek score 63.00
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
678
variable_sites
5
pi
0.00737
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CAGCTCCACACTTTCTTTCCT AAAGAAGCCATTGCGATTGC 47.6 45.0 58.1 58.0 791 bp 1.0 71.4
accD-psaI_p2 TCAGCTCCACACTTTCTTTCC AAAGAAGCCATTGCGATTGC 47.6 45.0 58.1 58.0 792 bp 1.0 71.3
accD-psaI_p3 AGCTCCACACTTTCTTTCCT AAAGAAGCCATTGCGATTGC 45.0 45.0 56.7 58.0 790 bp 1.0 69.8
accD-psaI_p4 CAGCTCCACACTTTCTTTCC AAAGAAGCCATTGCGATTGC 50.0 45.0 56.6 58.0 791 bp 1.0 69.6
accD-psaI_p5 CAGCTCCACACTTTCTTTCCT TTGCGATTGCCGGAAATACT 47.6 45.0 58.1 58.3 781 bp 1.0 69.3