Hotspot

atpF-atpA

LSC · 28309-28378 · 70 bp

Back to Dahlia

Pi 0.0154
MarkerSeek score 65.40
Primer available yes
Label atpF-atpA

Feature Metadata

label_name
atpF-atpA
feature_type
igs
region
LSC
length_bp
70
variable_sites
1
pi
0.0154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpA_p1 CATATTGGCGGCGATGAAAC GTCGGCTTGAATGGTTACCA 50.0 50.0 58.0 58.2 120-125 bp 1.0 82.2
atpF-atpA_p2 CATATTGGCGGCGATGAAAC ATTTCGTCGGCTTGAATGGT 50.0 45.0 58.0 58.2 125-130 bp 1.0 82.0
atpF-atpA_p3 CATATTGGCGGCGATGAAAC TCGTCGGCTTGAATGGTTAC 50.0 50.0 58.0 58.3 122-127 bp 1.0 81.3
atpF-atpA_p4 CATATTGGCGGCGATGAAAC TTTCGTCGGCTTGAATGGTT 50.0 45.0 58.0 58.4 124-129 bp 1.0 80.3
atpF-atpA_p5 CATATTGGCGGCGATGAAAC ACTTGAAGTACGGTACCGGT 50.0 50.0 58.0 58.4 203-208 bp 1.0 79.0