Hotspot

ycf1

SSC · 123099-128546 · 5448 bp

Back to Dactylorhiza

Pi 0.00731

MarkerSeek score 38.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5448
variable_sites: 75
pi: 0.00731
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCAAGGTTCTAGATTGGCA	TGAGATCTGGACCAATGCTG	45.0	50.0	56.4	57.3	191-203 bp	1.0	69.0
ycf1_p2	TGGGATGATCCAAGGTTGAA	TGAGATCTGGACCAATGCTG	45.0	50.0	56.4	57.3	250-262 bp	1.0	67.8
ycf1_p3	TTGGGATGATCCAAGGTTGA	TGAGATCTGGACCAATGCTG	45.0	50.0	56.4	57.3	251-263 bp	1.0	67.8
ycf1_p4	AACCAAGGTTCTAGATTGGCA	TGAGATCTGGACCAATGCTG	42.9	50.0	57.2	57.3	192-204 bp	1.0	67.1
ycf1_p5	TTGGGATGATCCAAGGTTGAA	TGAGATCTGGACCAATGCTG	42.9	50.0	57.2	57.3	251-263 bp	1.0	66.0