Hotspot

rps15

SSC · 122465-122737 · 273 bp

Back to Dactylorhiza

Pi 0.00147

MarkerSeek score 25.60

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 1
pi: 0.00147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GATCAGCCGAAGAACACCAT	TAAGAAACTGCGGGCAAGTT	50.0	45.0	58.0	58.0	1108-1118 bp	1.0	82.3
rps15_p2	AACACCATGCATTGATGGGT	TAAGAAACTGCGGGCAAGTT	45.0	45.0	58.1	58.0	1096-1106 bp	1.0	82.1
rps15_p3	TTTCCAATCGTTCTGGTGCA	TAAGAAACTGCGGGCAAGTT	45.0	45.0	58.0	58.0	1292-1302 bp	1.0	81.9
rps15_p4	GATCAGCCGAAGAACACCAT	TTTCACTAGGAGGAGCGGAA	50.0	50.0	58.0	58.1	775-785 bp	0.8	73.7
rps15_p5	TTTCCAATCGTTCTGGTGCA	TTTCACTAGGAGGAGCGGAA	45.0	50.0	58.0	58.1	959-969 bp	0.8	73.2