Hotspot

rpoC1

LSC · 19867-22658 · 2792 bp

Back to Dactylorhiza

Pi 0.00358

MarkerSeek score 59.90

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2792
variable_sites: 20
pi: 0.00358
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	ACACGAGTATGGGCATCACT	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.8	58.0	2942-2974 bp	1.0	74.6
rpoC1_p2	TACACGAGTATGGGCATCAC	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.1	58.0	2943-2975 bp	1.0	74.0
rpoC1_p3	TTACACGAGTATGGGCATCAC	TTGCTCGTTCGAGAACTACG	47.6	50.0	57.8	58.0	2944-2976 bp	1.0	71.7
rpoC1_p4	CACGAGTATGGGCATCACTA	TTGCTCGTTCGAGAACTACG	50.0	50.0	56.8	58.0	2941-2973 bp	1.0	71.7
rpoC1_p5	ACCAGTTAAATCGGGGAGAC	TTGCTCGTTCGAGAACTACG	50.0	50.0	56.9	58.0	2970-3000 bp	0.6	56.2