Hotspot

infA

LSC · 78960-79193 · 234 bp

Back to Dactylorhiza

Pi 0

MarkerSeek score 19.40

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 234
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	CAGAAGCCCAAGAAACCACT	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.0	58.0	1020-1026 bp	1.0	81.8
infA_p2	TCATAACCTCTGCTCGTTGC	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.0	58.0	1137-1143 bp	1.0	81.7
infA_p3	GAAACCACTCGACCTCGTAC	GGCGGAATGGGGATTGTAAT	55.0	50.0	58.0	58.0	1009-1015 bp	1.0	81.7
infA_p4	CAGAAGCCCAAGAAACCACT	TTTAGGCGGAATGGGGATTG	50.0	50.0	58.0	57.9	1024-1030 bp	1.0	81.3
infA_p5	TCATAACCTCTGCTCGTTGC	TTTAGGCGGAATGGGGATTG	50.0	50.0	58.0	57.9	1141-1147 bp	1.0	81.2