Hotspot

ccsA

SSC · 113704-114681 · 978 bp

Back to Dactylorhiza

Pi 0.00542

MarkerSeek score 62.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 9
pi: 0.00542
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CGAGTGGCGGCATATATAAT	CCCCATTCAAACGGTTTTCA	45.0	45.0	55.3	56.8	1170 bp	1.0	49.3
ccsA_p2	CGAGTGGCGGCATATATAAT	TCCCCATTCAAACGGTTTTCA	45.0	42.9	55.3	58.3	1171 bp	1.0	48.2
ccsA_p3	CGAGTGGCGGCATATATAAT	CCCCATTCAAACGGTTTTCAA	45.0	42.9	55.3	57.5	1170 bp	1.0	47.1
ccsA_p4	CGAGTGGCGGCATATATAAT	TCCCCATTCAAACGGTTTTC	45.0	45.0	55.3	56.5	1171 bp	1.0	47.0
ccsA_p5	CGAGTGGCGGCATATATAAT	CGGTTTTCAAAAACTCCCACA	45.0	42.9	55.3	57.5	1159 bp	1.0	46.7