Hotspot

ndhH-rps15

SSC · 122322-122464 · 143 bp

Back to Dactylorhiza

Pi 0.0266

MarkerSeek score 62.50

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 143
variable_sites: 7
pi: 0.0266
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	TCTTGTAGCCGGTACAGTCA	TTCTGGGAAAACGTCAACGA	50.0	45.0	58.1	57.7	269-273 bp	1.0	80.6
ndhH-rps15_p2	GATCAGCCGAAGAACACCAT	TTCTGGGAAAACGTCAACGA	50.0	45.0	58.0	57.7	335-339 bp	1.0	80.3
ndhH-rps15_p3	AACACCATGCATTGATGGGT	TTCTGGGAAAACGTCAACGA	45.0	45.0	58.1	57.7	323-327 bp	1.0	80.2
ndhH-rps15_p4	ATGCATTGATGGGTGGTGAG	TTCTGGGAAAACGTCAACGA	50.0	45.0	58.2	57.7	317-321 bp	1.0	78.9
ndhH-rps15_p5	GATCAGCCGAAGAACACCAT	TCTGGGAAAACGTCAACGAT	50.0	45.0	58.0	57.5	334-338 bp	1.0	78.6