Hotspot

rpl16-rps3

LSC · 82003-82181 · 179 bp

Back to Dactylorhiza

Pi 0.0283

MarkerSeek score 64.00

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 10
pi: 0.0283
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AACGAGTCACACACTAAGCA	AGAGAAGGCAGAGTTCCCTT	45.0	50.0	57.1	58.0	314-324 bp	1.0	77.1
rpl16-rps3_p2	ACGAGTCACACACTAAGCAT	AGAGAAGGCAGAGTTCCCTT	45.0	50.0	56.9	58.0	313-323 bp	1.0	75.3
rpl16-rps3_p3	AGTTTATAGTGGGGTCAGCCT	AGAGAAGGCAGAGTTCCCTT	47.6	50.0	58.2	58.0	382-389 bp	1.0	74.8
rpl16-rps3_p4	AACGAGTCACACACTAAGCA	CCCTTACAAACAATTCGCGC	45.0	50.0	57.1	58.4	299-309 bp	1.0	74.6
rpl16-rps3_p5	ATAGTGGGGTCAGCCTTTTT	AGAGAAGGCAGAGTTCCCTT	45.0	50.0	56.7	58.0	377-384 bp	1.0	73.7