Hotspot

petD-rpoA

LSC · 76854-77071 · 218 bp

Back to Dactylorhiza

Pi 0.0174

MarkerSeek score 57.40

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 218
variable_sites: 8
pi: 0.0174
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CGTCGTCCAGTAGCTACAAC	TTGGGCACTTTACAGAAGCA	55.0	45.0	58.1	57.6	356-357 bp	1.0	78.1
petD-rpoA_p2	TCGTCGTCCAGTAGCTACAA	TTGGGCACTTTACAGAAGCA	50.0	45.0	58.2	57.6	357-358 bp	1.0	77.4
petD-rpoA_p3	TTCGTCGTCCAGTAGCTACA	TTGGGCACTTTACAGAAGCA	50.0	45.0	58.2	57.6	358-359 bp	1.0	77.4
petD-rpoA_p4	CATTTCGTCGTCCAGTAGCT	TTGGGCACTTTACAGAAGCA	50.0	45.0	57.7	57.6	361-362 bp	1.0	76.7
petD-rpoA_p5	AATTGGTACCGCAGTAGCTC	TTGGGCACTTTACAGAAGCA	50.0	45.0	57.7	57.6	327-328 bp	1.0	76.6