Hotspot

trnP-UGG-psaJ

LSC · 64824-65186 · 363 bp

Back to Dactylorhiza

Pi 0.013

MarkerSeek score 46.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 363
variable_sites: 8
pi: 0.013
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GTGCCGTGGAGAGATATGTT	50.0	50.0	58.1	57.4	443-462 bp	1.0	77.7
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GTGCCGTGGAGAGATATGTT	50.0	50.0	58.1	57.4	442-461 bp	1.0	77.7
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GTGCCGTGGAGAGATATGTT	50.0	50.0	58.1	57.4	454-473 bp	1.0	77.6
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GTGCCGTGGAGAGATATGTT	50.0	50.0	58.1	57.4	453-472 bp	1.0	77.6
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GTGCCGTGGAGAGATATGTT	50.0	50.0	58.1	57.4	456-475 bp	1.0	77.5