Hotspot

rbcL-accD

LSC · 55470-56229 · 760 bp

Back to Dactylorhiza

Pi 0.0107

MarkerSeek score 56.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 15
pi: 0.0107
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACTACAGTTCGGTGGAGGAA	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.0	58.0	1090-1111 bp	1.0	83.0
rbcL-accD_p2	ACTACAGTTCGGTGGAGGAA	GTCCAAGAGCATCAGCATCA	50.0	50.0	58.0	58.0	1080-1101 bp	1.0	83.0
rbcL-accD_p3	ACTACAGTTCGGTGGAGGAA	TGTCCAAGAGCATCAGCATC	50.0	50.0	58.0	58.0	1081-1102 bp	1.0	83.0
rbcL-accD_p4	ACTACAGTTCGGTGGAGGAA	ACTGCCCCAACTAGGAATCT	50.0	50.0	58.0	58.0	1145-1166 bp	1.0	82.9
rbcL-accD_p5	GGCTTCAGGGGGTATTCATG	ACTGCCCCAACTAGGAATCT	55.0	50.0	58.0	58.0	1211-1232 bp	1.0	82.7