Hotspot

trnC-GCA-petN

LSC · 27212-28092 · 881 bp

Back to Dactylorhiza

Pi 0.00821

MarkerSeek score 60.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 881
variable_sites: 13
pi: 0.00821
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTGGATGAAATTTGGCGGC	CCCACTCCTTCCCCATACTA	45.0	55.0	57.9	57.5	672-1060 bp	1.0	80.4
trnC-GCA-petN_p2	CCAGTTCGAATCTGGGTGTC	CCCACTCCTTCCCCATACTA	55.0	55.0	58.3	57.5	611-999 bp	1.0	79.0
trnC-GCA-petN_p3	TTTGGATGAAATTTGGCGGC	TCTAAAGCCCACTCCTTCCC	45.0	55.0	57.9	58.7	679-1067 bp	1.0	78.6
trnC-GCA-petN_p4	GATGAAATTTGGCGGCATGG	CCCACTCCTTCCCCATACTA	50.0	55.0	58.4	57.5	668-1056 bp	1.0	77.7
trnC-GCA-petN_p5	GGATGAAATTTGGCGGCATG	CCCACTCCTTCCCCATACTA	50.0	55.0	58.4	57.5	669-1057 bp	1.0	77.7