Hotspot

trnS-GCU-trnG-GCC

LSC · 7922-8545 · 624 bp

Back to Dactylorhiza

Pi 0.0159

MarkerSeek score 66.40

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 624
variable_sites: 17
pi: 0.0159
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	654-679 bp	1.0	66.4
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	653-678 bp	1.0	66.4
trnS-GCU-trnG-GCC_p3	ATCGGAGAGAGAGGGATTCG	ACCACTAAACTATACCCGCT	55.0	45.0	58.1	55.6	709-734 bp	1.0	65.3
trnS-GCU-trnG-GCC_p4	GGAATCGGAGAGAGAGGGAT	ACCACTAAACTATACCCGCT	55.0	45.0	57.7	55.6	712-737 bp	1.0	63.6
trnS-GCU-trnG-GCC_p5	CGGAGAGAGAGGGATTCGAA	ACCACTAAACTATACCCGCT	55.0	45.0	58.3	55.6	707-732 bp	1.0	63.5