Hotspot

C2H24_pgr004-C2H24_pgr003

IRa · 116588-116814 · 227 bp

Back to Dactyloctenium

Pi 0.0441

MarkerSeek score 77.80

Primer available yes

Label C2H24_pgr004-C2H24_pgr003

Feature Metadata

label_name: C2H24_pgr004-C2H24_pgr003
feature_type: igs
region: IRa
length_bp: 227
variable_sites: 10
pi: 0.0441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
C2H24_pgr004-C2H24_pgr003_p1	AGTATCGTCACCGCAGTAGA	AGGCATCCTAACGAACGAAC	50.0	50.0	58.0	58.0	337 bp	1.0	82.1
C2H24_pgr004-C2H24_pgr003_p2	TATCGTCACCGCAGTAGAGT	AGGCATCCTAACGAACGAAC	50.0	50.0	58.0	58.0	335 bp	1.0	82.1
C2H24_pgr004-C2H24_pgr003_p3	ATTCGGGATGGATTGGTGTG	AGGCATCCTAACGAACGAAC	50.0	50.0	57.9	58.0	304 bp	1.0	82.1
C2H24_pgr004-C2H24_pgr003_p4	CCACCAAATTCGGGATGGAT	AGGCATCCTAACGAACGAAC	50.0	50.0	57.9	58.0	311 bp	1.0	82.0
C2H24_pgr004-C2H24_pgr003_p5	TCCCCTACAGTATCGTCACC	AGGCATCCTAACGAACGAAC	55.0	50.0	57.9	58.0	345 bp	1.0	81.8