Hotspot

psaJ-rpl33

LSC · 64797-65214 · 418 bp

Back to Dactyloctenium

Pi 0.0148

MarkerSeek score 66.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 418
variable_sites: 6
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTGGGGCTTTAGCAGGTTT	CTAGAAATCCCCGCCGATTC	45.0	55.0	57.9	58.2	576-588 bp	1.0	80.1
psaJ-rpl33_p2	TTTGGGGCTTTAGCAGGTTT	TATCTAGAAATCCCCGCCGA	45.0	50.0	57.9	57.7	579-591 bp	1.0	79.3
psaJ-rpl33_p3	TTTGGGGCTTTAGCAGGTTT	ATCTAGAAATCCCCGCCGAT	45.0	50.0	57.9	58.4	578-590 bp	1.0	78.9
psaJ-rpl33_p4	TTTGGGGCTTTAGCAGGTTT	CCGCCGATTCCTTATTGGTA	45.0	50.0	57.9	57.5	566-578 bp	1.0	77.5
psaJ-rpl33_p5	ATGGTTTGGGGCTTTAGCAG	CTAGAAATCCCCGCCGATTC	50.0	55.0	58.4	58.2	580-592 bp	1.0	77.3