Hotspot

psbE-petL

LSC · 62307-63492 · 1186 bp

Back to Dactyloctenium

Pi 0.0144

MarkerSeek score 67.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1186
variable_sites: 17
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.0	58.5	1344-1448 bp	1.0	77.3
psbE-petL_p2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.0	58.5	1342-1446 bp	1.0	77.3
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1333-1437 bp	1.0	77.1
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1331-1435 bp	1.0	77.0
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.2	58.5	1335-1439 bp	1.0	75.6