Hotspot

rbcL-psaI

LSC · 56494-57370 · 877 bp

Back to Dactyloctenium

Pi 0.0183

MarkerSeek score 57.50

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 877
variable_sites: 16
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CATTCGACTTCAAGCCGGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	982-992 bp	1.0	81.4
rbcL-psaI_p2	AAGCCGGTAGATACCATCGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	971-981 bp	1.0	81.4
rbcL-psaI_p3	CAAGCCGGTAGATACCATCG	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.9	58.0	972-982 bp	1.0	81.0
rbcL-psaI_p4	CATTCGACTTCAAGCCGGTA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	988-998 bp	1.0	80.8
rbcL-psaI_p5	AAGCCGGTAGATACCATCGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	977-987 bp	1.0	80.8