Hotspot

trnG-GCC-trnfM-CAU

LSC · 12540-12915 · 376 bp

Back to Dactyloctenium

Pi 0.0324
MarkerSeek score 64.80
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
376
variable_sites
12
pi
0.0324
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 GAAGGTACGGGTTCGATTCC AGTTTTTGGCCCTACAGAGC 55.0 50.0 58.1 58.1 498-510 bp 1.0 81.6
trnG-GCC-trnfM-CAU_p2 GCGAGCGTAGTTCAATGGTA AGTTTTTGGCCCTACAGAGC 50.0 50.0 58.1 58.1 538-550 bp 1.0 81.3
trnG-GCC-trnfM-CAU_p3 GAAGGTACGGGTTCGATTCC CGAGGCTCATAACCTTGAGG 55.0 55.0 58.1 57.8 454-466 bp 1.0 80.7
trnG-GCC-trnfM-CAU_p4 GCGAGCGTAGTTCAATGGTA CGAGGCTCATAACCTTGAGG 50.0 55.0 58.1 57.8 494-506 bp 1.0 80.4
trnG-GCC-trnfM-CAU_p5 TAGCGAGCGTAGTTCAATGG AGTTTTTGGCCCTACAGAGC 50.0 50.0 57.8 58.1 540-552 bp 1.0 80.3