Hotspot

rps16

LSC · 4514-5599 · 1086 bp

Back to Dactylis

Pi 0.000921

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1086
variable_sites: 1
pi: 0.000921
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGGCACGACATAAATCCAAA	GCTCCATAGACAGCAAGTTTG	40.0	47.6	55.6	57.2	1326-1327 bp	1.0	47.2
rps16_p2	ACTAGTGATCTTCTCCACTCCT	GCTCCATAGACAGCAAGTTTG	45.5	47.6	57.4	57.2	1255-1256 bp	1.0	45.2
rps16_p3	AGTGATCTTCTCCACTCCTACT	GCTCCATAGACAGCAAGTTTG	45.5	47.6	57.4	57.2	1252-1253 bp	1.0	45.2
rps16_p4	TGGCACGACATAAATCCAAAT	GCTCCATAGACAGCAAGTTTG	38.1	47.6	56.2	57.2	1326-1327 bp	1.0	43.7
rps16_p5	TGGCACGACATAAATCCAAA	GCTCCATAGACAGCAAGTTT	40.0	45.0	55.6	55.7	1326-1327 bp	1.0	43.1