Hotspot

rpoA

LSC · 73418-74440 · 1023 bp

Back to Dactylis

Pi 0.000978

MarkerSeek score 59.30

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1023
variable_sites: 1
pi: 0.000978
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AGATTGTGCCAAAGATGCAA	ACACCTATGCCGCATAATGG	40.0	50.0	56.2	58.1	1224 bp	1.0	64.9
rpoA_p2	AGATTGTGCCAAAGATGCAA	CACCTATGCCGCATAATGGA	40.0	50.0	56.2	57.8	1223 bp	1.0	64.2
rpoA_p3	AGATTGTGCCAAAGATGCAA	GGATGTCGACCGCCTAAAAA	40.0	50.0	56.2	58.3	1206 bp	1.0	63.5
rpoA_p4	AGATTGTGCCAAAGATGCAA	TAATGGATGTCGACCGCCTA	40.0	50.0	56.2	58.3	1210 bp	1.0	63.3
rpoA_p5	AGATTGTGCCAAAGATGCAAA	ACACCTATGCCGCATAATGG	38.1	50.0	57.0	58.1	1224 bp	1.0	63.0