Hotspot

matK

LSC · 1687-3222 · 1536 bp

Back to Dactylis

Pi 0.0013

MarkerSeek score 59.40

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1536
variable_sites: 2
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	AAGTGGATTCGCCTACTCTT	CAACACCCTGTTCTGACCAT	45.0	50.0	56.3	57.7	1740 bp	1.0	63.9
matK_p2	AGTGGATTCGCCTACTCTTT	CAACACCCTGTTCTGACCAT	45.0	50.0	56.3	57.7	1739 bp	1.0	63.9
matK_p3	AAGTGGATTCGCCTACTCTTT	CAACACCCTGTTCTGACCAT	42.9	50.0	57.0	57.7	1740 bp	1.0	62.0
matK_p4	AAGTGGATTCGCCTACTCTT	ATCCAACACCCTGTTCTGAC	45.0	50.0	56.3	57.4	1743 bp	1.0	61.6
matK_p5	AGTGGATTCGCCTACTCTTT	ATCCAACACCCTGTTCTGAC	45.0	50.0	56.3	57.4	1742 bp	1.0	61.6