Hotspot

rbcL-psaI

LSC · 55178-56405 · 1228 bp

Back to Dactylis

Pi 0.00572

MarkerSeek score 62.40

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1228
variable_sites: 7
pi: 0.00572
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AGTTCGAGCCGGTAGATACT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.7	58.0	1330-1547 bp	1.0	77.7
rbcL-psaI_p2	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	1344-1561 bp	1.0	77.6
rbcL-psaI_p3	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.6	58.0	1349-1566 bp	1.0	77.3
rbcL-psaI_p4	AGTTCGAGCCGGTAGATACT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.7	57.9	1336-1553 bp	1.0	77.1
rbcL-psaI_p5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.6	57.9	1350-1567 bp	1.0	77.0