Hotspot

ndhC-trnV-UAC

LSC · 49153-49990 · 838 bp

Back to Dactylis

Pi 0.00239

MarkerSeek score 60.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 2
pi: 0.00239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCCAGAAATCCAAAATGCC	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.0	57.9	948-949 bp	1.0	79.3
ndhC-trnV-UAC_p2	GCCCAGAAATCCAAAATGCC	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.0	58.3	949-950 bp	1.0	77.5
ndhC-trnV-UAC_p3	CTCTGGTCCTTCACTAACCG	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.3	57.9	977-978 bp	1.0	74.3
ndhC-trnV-UAC_p4	TCTGGTCCTTCACTAACCGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.7	57.9	976-977 bp	1.0	73.7
ndhC-trnV-UAC_p5	TCACTAACCGGGGCTAAAAG	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.2	57.9	967-968 bp	1.0	73.4