Hotspot

psaA-ycf3

LSC · 40427-41085 · 659 bp

Back to Dactylis

Pi 0.00152

MarkerSeek score 59.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 1
pi: 0.00152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	855 bp	1.0	77.3
psaA-ycf3_p2	GTTTTTGAGAAATGGCCGGG	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.9	57.7	874 bp	1.0	77.3
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAA	GCTATAGCGCTTACTCCAGG	50.0	55.0	58.1	57.6	822 bp	1.0	76.6
psaA-ycf3_p4	GTTTTTGAGAAATGGCCGGG	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	841 bp	1.0	76.6
psaA-ycf3_p5	CTGGCCCATTCCTCAAAAGA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.8	57.7	853 bp	1.0	76.1