Hotspot

rps16-trnQ-UUG

LSC · 5600-6368 · 769 bp

Back to Dactylis

Pi 0.0013
MarkerSeek score 59.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
769
variable_sites
1
pi
0.0013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 842 bp 1.0 78.4
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 853 bp 1.0 77.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 846 bp 1.0 77.3
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 857 bp 1.0 76.8
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TACTCGGAGGTTCGAATCCT 45.0 50.0 58.1 57.6 848 bp 1.0 75.5