Hotspot

trnK-UUU-rps16

LSC · 3955-4513 · 559 bp

Back to Dactylis

Pi 0.00358
MarkerSeek score 45.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
559
variable_sites
2
pi
0.00358
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TGGACGAATTTGTTGCTCCA TCGAGCCGTATGAGGAGAAA 45.0 50.0 58.0 58.2 935-943 bp 1.0 78.1
trnK-UUU-rps16_p2 TGGACGAATTTGTTGCTCCA TTTCTCGAGCCGTATGAGGA 45.0 50.0 58.0 58.2 939-947 bp 1.0 78.1
trnK-UUU-rps16_p3 TGAGGATCAGTCGTGGTCTT TCGAGCCGTATGAGGAGAAA 50.0 50.0 58.1 58.2 974-982 bp 1.0 77.7
trnK-UUU-rps16_p4 TGAGGATCAGTCGTGGTCTT TTTCTCGAGCCGTATGAGGA 50.0 50.0 58.1 58.2 978-986 bp 1.0 77.7
trnK-UUU-rps16_p5 TTTTTGCAGGCCAATTGCTC TCGAGCCGTATGAGGAGAAA 45.0 50.0 58.1 58.2 1369-1377 bp 1.0 77.5