Hotspot

ycf3

LSC · 45019-46968 · 1950 bp

Back to Dactyladenia

Pi 0.00274

MarkerSeek score 59.90

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1950
variable_sites: 8
pi: 0.00274
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	CTCAAAGAGAGTGTTCTTAGC	TTGTGGGGTCGTTTTCTCAC	42.9	50.0	54.0	58.3	2031-2035 bp	1.0	40.6
ycf3_p2	CTCAAAGAGAGTGTTCTTAGC	TTTGTGGGGTCGTTTTCTCA	42.9	45.0	54.0	57.6	2032-2036 bp	1.0	40.6
ycf3_p3	TCAAAGAGAGTGTTCTTAGC	TTGTGGGGTCGTTTTCTCAC	40.0	50.0	52.6	58.3	2030-2034 bp	1.0	40.6
ycf3_p4	TCAAAGAGAGTGTTCTTAGC	TTTGTGGGGTCGTTTTCTCA	40.0	45.0	52.6	57.6	2031-2035 bp	1.0	40.6
ycf3_p5	CTCAAAGAGAGTGTTCTTAGC	TTTTGTGGGGTCGTTTTCTCA	42.9	42.9	54.0	58.3	2033-2037 bp	1.0	40.6