Hotspot

ycf1

SSC · 130816-136536 · 5721 bp

Back to Dactyladenia

Pi 0.00326

MarkerSeek score 50.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5721
variable_sites: 28
pi: 0.00326
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAGAAGGTTTTGGATGAACC	CTTGGCACAGATCGAAGTCA	40.0	50.0	52.8	57.9	293 bp	1.0	41.1
ycf1_p2	TAGAAGGTTTTGGATGAACC	AACCTTGGCACAGATCGAAG	40.0	50.0	52.8	58.2	296 bp	1.0	41.1
ycf1_p3	TAGAAGGTTTTGGATGAACC	AAAACCTTGGCACAGATCGA	40.0	45.0	52.8	57.7	298 bp	1.0	41.1
ycf1_p4	TAGAAGGTTTTGGATGAACC	AAACCTTGGCACAGATCGAA	40.0	45.0	52.8	57.7	297 bp	1.0	41.1
ycf1_p5	TAGAAGGTTTTGGATGAACC	TGGCACAGATCGAAGTCAAA	40.0	45.0	52.8	57.5	291 bp	1.0	41.1