Hotspot

rpl20

LSC · 72703-73056 · 354 bp

Back to Dactyladenia

Pi 0.0113

MarkerSeek score 40.60

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 354
variable_sites: 6
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	CTCGGAATTCATTCTCCCGG	ACATTGGCAGAATCCGTTTTG	55.0	42.9	58.1	57.7	517-531 bp	1.0	69.5
rpl20_p2	CTCGGAATTCATTCTCCCGG	CATTGGCAGAATCCGTTTTGT	55.0	42.9	58.1	57.7	516-530 bp	1.0	69.5
rpl20_p3	CGGAATTCATTCTCCCGGAA	ACATTGGCAGAATCCGTTTTG	50.0	42.9	57.7	57.7	515-529 bp	1.0	67.9
rpl20_p4	CGGAATTCATTCTCCCGGAA	CATTGGCAGAATCCGTTTTGT	50.0	42.9	57.7	57.7	514-528 bp	1.0	67.9
rpl20_p5	CTCGGAATTCATTCTCCCGG	TTGGCAGAATCCGTTTTGTT	55.0	40.0	58.1	56.4	514-528 bp	1.0	67.4