Hotspot

rpl16

LSC · 85868-87337 · 1470 bp

Back to Dactyladenia

Pi 0.00544

MarkerSeek score 63.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1470
variable_sites: 12
pi: 0.00544
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AATTTTCGGGCTCCACTGTT	ACTCTTTCATTCCTCTCTGTCT	45.0	40.9	58.0	56.2	1665-1754 bp	1.0	51.0
rpl16_p2	AATTTTCGGGCTCCACTGTT	TCATTCCTCTCTGTCTTCAATCA	45.0	39.1	58.0	57.0	1659-1748 bp	1.0	49.3
rpl16_p3	AATTTTCGGGCTCCACTGTT	CTCTTTCATTCCTCTCTGTCTTCA	45.0	41.7	58.0	58.0	1664-1753 bp	1.0	49.0
rpl16_p4	AATTTTCGGGCTCCACTGTT	ACTCTTTCATTCCTCTCTGTCTTC	45.0	41.7	58.0	58.0	1665-1754 bp	1.0	49.0
rpl16_p5	AATTTTCGGGCTCCACTGTT	ACAAACTCTTTCATTCCTCTCTGT	45.0	37.5	58.0	58.1	1669-1758 bp	1.0	48.8