Hotspot

psbH

LSC · 78874-79095 · 222 bp

Back to Dactyladenia

Pi 0.003

MarkerSeek score 27.10

Primer available yes

Label psbH

Feature Metadata

label_name: psbH
feature_type: gene
region: LSC
length_bp: 222
variable_sites: 1
pi: 0.003
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH_p1	TGGTGCTCGAACTTTGTTCA	CCGTGCTCCAAATACTCGAA	45.0	50.0	58.0	58.0	1216 bp	1.0	80.4
psbH_p2	TGGTGCTCGAACTTTGTTCA	TTCCGTGCTCCAAATACTCG	45.0	50.0	58.0	58.0	1218 bp	1.0	80.4
psbH_p3	ACTGGGGGATCCAACTACAA	CCGTGCTCCAAATACTCGAA	50.0	50.0	57.9	58.0	1132 bp	1.0	80.2
psbH_p4	GTGTTCTTCGAAGGGATCCC	CCGTGCTCCAAATACTCGAA	55.0	50.0	58.0	58.0	713 bp	1.0	80.0
psbH_p5	GTGTTCTTCGAAGGGATCCC	TTCCGTGCTCCAAATACTCG	55.0	50.0	58.0	58.0	715 bp	1.0	80.0