Hotspot

petA

LSC · 65527-66489 · 963 bp

Back to Dactyladenia

Pi 0.00138

MarkerSeek score 59.60

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 2
pi: 0.00138
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	CAGCATCGTGGATAGGGAAC	ACTTGAATTTGATAAATCGGCA	55.0	31.8	58.1	54.7	1065 bp	1.0	40.2
petA_p2	GCATCGTGGATAGGGAACTC	ACTTGAATTTGATAAATCGGCA	55.0	31.8	57.8	54.7	1063 bp	1.0	40.2
petA_p3	CAGCATCGTGGATAGGGAAC	AACTTGAATTTGATAAATCGGCA	55.0	30.4	58.1	55.5	1066 bp	1.0	40.2
petA_p4	GCATCGTGGATAGGGAACTC	AACTTGAATTTGATAAATCGGCA	55.0	30.4	57.8	55.5	1064 bp	1.0	40.2
petA_p5	AACTCACCTAGTGCCCTACC	ACTTGAATTTGATAAATCGGCA	55.0	31.8	58.4	54.7	1048 bp	1.0	40.2