Hotspot

ndhA

SSC · 126491-128796 · 2306 bp

Back to Dactyladenia

Pi 0.00318

MarkerSeek score 52.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2306
variable_sites: 11
pi: 0.00318
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TTGTTTGAGACAAGACAGAA	GCCCACCGGGTTTTATCAAT	35.0	50.0	52.6	58.5	2449-2463 bp	1.0	40.8
ndhA_p2	TCTCTTGTTTTGTTTGAGACA	GCCCACCGGGTTTTATCAAT	33.3	50.0	53.5	58.5	2458-2472 bp	1.0	40.8
ndhA_p3	TCTCTTGTTTTGTTTGAGACAA	GCCCACCGGGTTTTATCAAT	31.8	50.0	54.4	58.5	2458-2472 bp	1.0	40.8
ndhA_p4	TTCTCTTGTTTTGTTTGAGACA	GCCCACCGGGTTTTATCAAT	31.8	50.0	54.4	58.5	2459-2473 bp	1.0	40.8
ndhA_p5	TGTTTGAGACAAGACAGAAT	GCCCACCGGGTTTTATCAAT	35.0	50.0	52.3	58.5	2448-2462 bp	1.0	40.8