Hotspot

ycf1-trnN-GUU

IRa · 136537-136913 · 377 bp

Back to Dactyladenia

Pi 0.00354

MarkerSeek score 36.90

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 377
variable_sites: 2
pi: 0.00354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	CCCATCGAGAATGTGGTCAG	ACGACCAGTCAGTTAACAGC	55.0	50.0	58.1	57.8	535-539 bp	1.0	79.3
ycf1-trnN-GUU_p2	CCCATCGAGAATGTGGTCAG	AGGATTCGAACCTACGACCA	55.0	50.0	58.1	58.2	548-552 bp	1.0	79.2
ycf1-trnN-GUU_p3	CCCATCGAGAATGTGGTCAG	GGATTCGAACCTACGACCAG	55.0	55.0	58.1	57.8	547-551 bp	1.0	78.8
ycf1-trnN-GUU_p4	ATAGAGTCCGACCACAACGA	ACGACCAGTCAGTTAACAGC	50.0	50.0	58.2	57.8	506-510 bp	1.0	78.4
ycf1-trnN-GUU_p5	ATAGAGTCCGACCACAACGA	AGGATTCGAACCTACGACCA	50.0	50.0	58.2	58.2	519-523 bp	1.0	78.3